Bio-Rad Got Protein? Kit

Product Details

Based on the Bio-Rad Quick Start™ Bradford protein assay, the Got Protein™ Kit is designed to introduce students to proteomics and provides the tools for them to develop their own protein-based experiments. In this laboratory activity, students use absorbance values from a set of protein samples with known concentrations to create a standard curve on linear graph paper. Protein concentrations of their test samples, such as milk, egg, sports drink, or saliva, can then be extrapolated by hand or plotted using a graphing utility such as Microsoft Excel software.

Features and Benefits

• Inquiry-based activity
• Integrates biology, chemistry, and physics in relation to protein structure and function

With this kit, students are able to:

• Measure protein concentrations
• Apply Beer’s law
• Learn to use a spectrophotometer
• Learn to use a micropipet

How It Works

The Bradford method is based on the color development formed when the dye Coomassie Blue G–250 binds to protein. There is a correlation between the amount of blue color and the amount of protein in the sample: the more protein, the more intense the blue color. The simplicity of the assay allows the results to be measured qualitatively by eye or quantitatively with a spectrophotometer.

More Information

• One 45 min laboratory activity
• Provides sufficient materials for eight student workstations, up to four students per workstation.

Bio-Rad Item No.: 1662900EDU

 

Please note that, though some items may be shipped at ambient temperatures to reduce shipping costs, some items are to be stored refrigerated or frozen upon receipt to ensure longevity. 

Please store some components at4°C as indicated.